How cytometer can Save You Time, Stress, and Money.
How cytometer can Save You Time, Stress, and Money.
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Data-enriched characterization of cell populations. Morphological information and facts from pictures can add to the richness of flow cytometry data. For example, the figure exhibits an if not traditional apoptosis assay applying Annexin V and PI, adding mobile imaging to characterize cells in Every single population to reveal morphologically unique options. These insights could not are gained from multiplex staining by itself.
Irrespective of whether you’re a novice or an expert, explore our resources to progress your investigate and abilities in flow cytometry.
Merge acoustic concentrating engineering with significant-parameter analysis for adaptable spectral unmixing and traditional workflows.
Uncover new insights into your experimental info by visualizing populations employing combined fluorescence and morphology details. Our program interface wizards help downsampling, concatenation (merged files) and dimensionality tools that happen to be designed to the software for an intuitive, streamlined information analysis encounter.
*Pricing is indicative only and is subject matter to alter. You should contact your Agilent representative with any queries. The information established forth herein is just not a proposal or a dedication, equally of that happen to be subject matter to credit score acceptance, conditions and terms, and availability with Agilent. Agilent Economical Remedies is offered in chosen international locations.
Also, it is actually illuminating to check out the quantity of doublets slip as a result of common singlet gates, and good to verify the position of unpredicted double-positive occasions in my Assessment."
Optimizing gating technique. Even strong manual singlet gating is mistake-susceptible and stays a subjective decision level in Nearly all flow cytometry assays. Imaging can be used to verify and change gates to include only solitary cells of desire.
Reliable impression high-quality even at substantial throughput Acoustic concentrating and also https://hoverboard01483.blog5.net/77476956/flow-cytometer-a-revolutionary-tool-for-cell-analysis a high-velocity digicam Incorporate to impression these T cells consistently at small or significant flow rates. Quickly change concentration and digicam options to meet experimental needs.
Early indications of this contamination had originally been dismissed as debris. Backgating to find out new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also enables you to use morphological attributes to discover interesting subpopulations that would not be apparent from flow cytometry facts by itself.
Maybe most of all, these occasions have cells of clearly distinctive phenotypes which may lead to incorrect conclusions regarding double constructive activities (particularly in rare populations).
AI-pushed automatic image Assessment Together with the Attune Cytometric Software interprets celebration capabilities into distinctive morphology parameters that could be combined with conventional fluorescence and scatter parameters to expose remarkably reproducible populace improvements and unique subsets.
Application Notice: Automatic impression Investigation decreases user-to-user variability in flow cytometry gating methods
Built-in dimensionality reduction equipment increased resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to confirm anticipated populace resolution in lysed total human blood. Consumer can see activities from Each individual cluster for visual affirmation of cell phenotypes.
Adaptable payment system from Agilent Financial Options assist you to get the vital instrumentation you will need for speedy project, or to improve your lab.
Gating on these prolonged parameters lets you speedily and accurately identify populations of interest and confirm gating technique with little or no guide overview. The Attune software package gives use of condition-of-the-art equipment Discovering analysis resources inside of an expertly created user interface, making it exceptionally simple to use.
The Attune Xenith cytometer is usually a high-parameter spectral analyzer, giving the two spectral unmixing and conventional flow capabilities for in depth and functional mobile Examination.
The Attune CytPix Flow Cytometer enables customers to visualize samples for the duration of acquisition, confirm the precision of gating methods, and aid effects with trusted documentation for publication uses.
What seemed to be a homogeneous inhabitants is in fact additional various—an Perception that should be regarded when interpreting final results.